Lemna Valdiviana

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Photosynthetic Mutant of Lemna

The biosynthesis of the cytochrome b6f complex has been studied in a mutant, no. 1073, of Lemna perpusilla that contained less than 1% of the four protein subunits when compared with a wild-type strain. RNA gel blot analyses of the mutant indicated that the chloroplast genes for cytochrome f , cytochrome b6, and subunit IV (petA, petB, and petD, respectively) are transcribed and that the petB a...

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Methionine methyl group metabolism in lemna.

To provide information upon the ways in which Lemna paucicostata uses the methyl group of methionine, plants were grown for various periods (from 1 minute to 6.8 days) in the presence of a tracer dose of radioactive methyl-labeled methionine. Protein methionine accounted for approximately 19% of the accumulated methyl moieties; other methylated products, about 81%. The latter group included (pe...

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Aspartokinase of Lemna paucicostata Hegelm. 6746.

A sensitive and specific method was developed for assay of aspartokinase (EC 2.7.2.4) in crude extracts of Lemna paucicostata. Lysine inhibited approximately 93%, and threonine approximately 6%; together, these amino acids inhibited 99%. Inhibition by lysine was synergistically increased by S-adenosylmethionine, which by itself had no effect on activity. Essentially complete inhibition of threo...

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C14-oxylipin glucosides isolated from Lemna paucicostata.

Oxylipin glucosides (2-4) were isolated from Lemna paucicostata with their structures and absolute configurations elucidated by spectroscopic and chemical methods. Compounds 2-4 were glucosides of C14 oxylipin which were synthesized from alpha-linolenic acid via the 9-lipoxygenase pathway.

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Threonine Synthase of Lemna paucicostata Hegelm. 6746.

Threonine synthase (TS) was purified approximately 40-fold from Lemna paucicostata, and some of its properties determined by use of a sensitive and specific assay. During the course of its purification, TS was separated from cystathionine gamma-synthase, establishing the separate identity of these enzymes. Compared to cystathionine gamma-synthase, TS is relatively insensitive to irreversible in...

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ژورنال

عنوان ژورنال: Botanical Gazette

سال: 1894

ISSN: 0006-8071

DOI: 10.1086/327131